FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9789118
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1139511.1640578855010226Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG699050.7141092793038147No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC345940.35339240981669645Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC304550.31111076605675814Illumina Single End PCR Primer 1 (100% over 49bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC249930.25531411512252683Illumina Single End PCR Primer 1 (100% over 47bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC247630.25296456739003453Illumina Single End PCR Primer 1 (100% over 49bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC237180.24228944834458016Illumina Single End PCR Primer 1 (100% over 47bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG222560.22735449710586797Illumina Single End PCR Primer 1 (100% over 48bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC218260.22296186438859966Illumina Single End PCR Primer 1 (100% over 49bp)
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC218000.22269626334057877Illumina Single End PCR Primer 1 (100% over 47bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC209460.2139722904555855Illumina Single End PCR Primer 1 (100% over 47bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG201950.20630050633775177Illumina Single End PCR Primer 1 (100% over 48bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG197350.2016014108727671Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT181080.1849809145216147Illumina Single End PCR Primer 1 (100% over 46bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC171520.17521496829438565Illumina Single End PCR Primer 1 (100% over 47bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG169820.17347834605732612Illumina Single End PCR Primer 1 (100% over 48bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC168590.1722218487916889Illumina Single End PCR Primer 1 (100% over 47bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG164800.16835020274553847Illumina Single End PCR Primer 1 (100% over 48bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC153610.15691914225571701Illumina Single End PCR Primer 1 (100% over 47bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC151420.15468196419738733Illumina Single End PCR Primer 1 (100% over 47bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG150900.1541507621013456Illumina Single End PCR Primer 1 (100% over 45bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT147990.15117807344849657Illumina Single End PCR Primer 1 (100% over 46bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG147750.15093290325032346Illumina Single End PCR Primer 1 (100% over 48bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT144680.14779676779869239Illumina Single End PCR Primer 1 (100% over 46bp)
GAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT140460.14348585848081513Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG139000.14199440644192868Illumina Single End PCR Primer 1 (100% over 45bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG135830.1387561167410588Illumina Single End PCR Primer 1 (100% over 48bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC132810.1356710584140471Illumina Single End PCR Primer 1 (100% over 47bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC132750.13560976586450385Illumina Single End PCR Primer 1 (100% over 47bp)
AAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT125530.12823422906946264Illumina Single End PCR Primer 1 (100% over 46bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC121680.12430129047376895Illumina Single End PCR Primer 1 (100% over 47bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG121640.12426042877407342Illumina Single End PCR Primer 1 (100% over 45bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC121360.12397439687620478Illumina Single End PCR Primer 1 (100% over 47bp)
CGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG121340.12395396602635701Illumina Single End PCR Primer 1 (100% over 45bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC120770.12337168680569587Illumina Single End PCR Primer 1 (100% over 47bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC120550.12314694745737052Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG118360.12090976939904084Illumina Single End PCR Primer 1 (100% over 48bp)
AGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT117500.12003124285558721Illumina Single End PCR Primer 1 (100% over 46bp)
GGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT114770.11724243185136801Illumina Single End PCR Primer 1 (100% over 46bp)
GAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT109920.11228795076328633Illumina Single End PCR Primer 1 (100% over 46bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG108040.11036745087759695Illumina Single End PCR Primer 1 (100% over 48bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC107880.11020400407881485Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT105740.1080179031451046Illumina Single End PCR Primer 1 (100% over 46bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG105110.10737433137490017Illumina Single End PCR Primer 1 (100% over 45bp)
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC105060.10732325425028078Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT101520.10370699382722733Illumina Single End PCR Primer 1 (100% over 46bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG101420.10360483957798854Illumina Single End PCR Primer 1 (100% over 48bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC101180.10335966937981543Illumina Single End PCR Primer 1 (100% over 47bp)
AAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT101130.10330859225519602Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG99910.10206231041448269Illumina Single End PCR Primer 1 (100% over 45bp)
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC98990.10112249132148575Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)