FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6A_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6A_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7681443
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1102201.434886648250856No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1070961.3942172063243847Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC523790.6818901084080166Illumina Single End PCR Primer 1 (100% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC296700.38625555120307475Illumina Single End PCR Primer 1 (100% over 49bp)
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGG270990.35278527745372845No Hit
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTTTTTTATTTCTTTCTAAGT170050.22137767604342048No Hit
AAAGGTTGTTTTATAGTAGTTAGGATGTAGAAAATGTATTCCGATAGGCC138650.1804999399201426No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC128450.1672211848737275Illumina Single End PCR Primer 1 (100% over 49bp)
GGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGGA99430.12944182492794648No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC97270.12662985327105858Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC92030.11980821832564532Illumina Single End PCR Primer 1 (100% over 49bp)
TAATTGGCATATTTGCATGACCTGTCCCACACAACTCAGAACATGCTCCA91650.11931351960822985No Hit
GGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC89220.11615005149423097No Hit
GGTGGGAGTGAGAGGTGTTATGGGTGGAGGACAATTTTTATTATATTTCA87670.11413220146266789No Hit
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG82410.10728452974265383Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)