FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6A_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6A_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751343
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG242191.3828815942964914No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC82170.4691827928623919Illumina Single End PCR Primer 1 (100% over 50bp)
AAAGGTTGTTTTATAGTAGTTAGGATGTAGAAAATGTATTCCGATAGGCC65050.37142924030301316No Hit
GGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC51930.29651530282760147No Hit
GGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATGA28180.16090508826654745No Hit
TGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGG24790.1415485144828854No Hit
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTTTTTTATTTCTTTCTAAGT24410.13937875104990854No Hit
GGTGGGAGTGAGAGGTGTTATGGGTGGAGGACAATTTTTATTATATTTCA23660.13509632322166476No Hit
GGTGGCAGGCATAGTAAAATGATGGTGTGGAAGACATAGATGGTATTTGT19950.11391258023128537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)