FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6A_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6A_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18147501
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1816961.0012177434237364Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1784610.9833915975538451No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC991610.5464168317169399Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC905320.49886758512921425Illumina Single End PCR Primer 1 (100% over 49bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG858790.4732276912396919Illumina Single End PCR Primer 1 (100% over 48bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC850890.4688744747830569Illumina Single End PCR Primer 1 (100% over 49bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG819960.4518308057952442Illumina Single End PCR Primer 1 (100% over 48bp)
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC816980.4501887064229946Illumina Single End PCR Primer 1 (100% over 47bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC802310.4421049487750407Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG767210.4227634427461941Illumina Single End PCR Primer 1 (100% over 48bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC760780.4192202551745279Illumina Single End PCR Primer 1 (100% over 47bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC734120.4045295272335293Illumina Single End PCR Primer 1 (100% over 47bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC682840.3762721930694479Illumina Single End PCR Primer 1 (100% over 47bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG588760.3244303444314454Illumina Single End PCR Primer 1 (100% over 48bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC579600.31938281750198005Illumina Single End PCR Primer 1 (100% over 49bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC565950.3118611207129841Illumina Single End PCR Primer 1 (100% over 47bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG548610.3023060861106992Illumina Single End PCR Primer 1 (100% over 48bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC536120.2954235957887535Illumina Single End PCR Primer 1 (100% over 47bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG522570.2879570030055378Illumina Single End PCR Primer 1 (100% over 48bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG522010.28764842057316875Illumina Single End PCR Primer 1 (100% over 48bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC519520.28627633082924203Illumina Single End PCR Primer 1 (100% over 47bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC503690.27755336671423797Illumina Single End PCR Primer 1 (100% over 47bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC501570.2763851617916979Illumina Single End PCR Primer 1 (100% over 47bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG500500.2757955489298499Illumina Single End PCR Primer 1 (100% over 48bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG489790.2698939099107916Illumina Single End PCR Primer 1 (100% over 48bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC479370.26415207250849576Illumina Single End PCR Primer 1 (100% over 47bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC456910.2517757128102652Illumina Single End PCR Primer 1 (100% over 47bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC455080.2507673095044877Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG445870.24569223057213221Illumina Single End PCR Primer 1 (100% over 48bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC438360.24155391973803997Illumina Single End PCR Primer 1 (100% over 47bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC423350.23328280847043345Illumina Single End PCR Primer 1 (100% over 47bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT417300.229949016120732Illumina Single End PCR Primer 1 (100% over 46bp)
ACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC415180.22878081119819196Illumina Single End PCR Primer 1 (100% over 47bp)
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC396070.2182504356935977Illumina Single End PCR Primer 1 (100% over 47bp)
GATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC393560.21686732514851495Illumina Single End PCR Primer 1 (100% over 47bp)
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC377590.20806721542541862Illumina Single End PCR Primer 1 (100% over 47bp)
ATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC372120.20505302630924224Illumina Single End PCR Primer 1 (100% over 47bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC369280.20348807254508486Illumina Single End PCR Primer 1 (100% over 47bp)
TAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC361650.19928363690405637Illumina Single End PCR Primer 1 (100% over 47bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG361460.19917893929307404Illumina Single End PCR Primer 1 (100% over 48bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT357400.1969417166583983Illumina Single End PCR Primer 1 (100% over 46bp)
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG353180.1946163276144743Illumina Single End PCR Primer 1 (100% over 48bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT351730.19381731953066156Illumina Single End PCR Primer 1 (100% over 46bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC348880.19224685536592614Illumina Single End PCR Primer 1 (100% over 47bp)
GAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT326900.18013499489544044Illumina Single End PCR Primer 1 (100% over 46bp)
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT323860.17845983311972266Illumina Single End PCR Primer 1 (100% over 46bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC312070.17196307083823828Illumina Single End PCR Primer 1 (98% over 50bp)
AGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT301730.16626531664056665Illumina Single End PCR Primer 1 (100% over 46bp)
GAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC277830.15309545925910129Illumina Single End PCR Primer 1 (100% over 47bp)
CAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC270910.14928226205911216Illumina Single End PCR Primer 1 (100% over 47bp)
ATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC252290.13902189618284083Illumina Single End PCR Primer 1 (100% over 47bp)
GTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC251100.13836615851405656Illumina Single End PCR Primer 1 (100% over 47bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG249800.1376498064389141Illumina Single End PCR Primer 1 (100% over 45bp)
AAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT248170.136751611144697Illumina Single End PCR Primer 1 (100% over 46bp)
GCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC245000.13500481416146498Illumina Single End PCR Primer 1 (100% over 47bp)
GTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC244290.13461357572042565Illumina Single End PCR Primer 1 (100% over 47bp)
AAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT243200.13401294205742156Illumina Single End PCR Primer 1 (100% over 46bp)
GTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC241750.1332139339736088Illumina Single End PCR Primer 1 (100% over 47bp)
TGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC237670.13096569053777707Illumina Single End PCR Primer 1 (100% over 47bp)
CAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC228450.12588510120484356Illumina Single End PCR Primer 1 (100% over 47bp)
GCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC228030.12565366438056677Illumina Single End PCR Primer 1 (100% over 47bp)
AGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT222750.12274417287537276Illumina Single End PCR Primer 1 (100% over 46bp)
ATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC222310.12250171524993993Illumina Single End PCR Primer 1 (100% over 47bp)
ACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC215670.11884280926613533Illumina Single End PCR Primer 1 (100% over 47bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT214000.11792257236960615Illumina Single End PCR Primer 1 (100% over 46bp)
AGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT213980.11791155156845011Illumina Single End PCR Primer 1 (100% over 46bp)
ACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC213470.11763052113897114Illumina Single End PCR Primer 1 (100% over 47bp)
TGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC205740.11337098149216246Illumina Single End PCR Primer 1 (100% over 47bp)
GAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT202400.11153050769910414Illumina Single End PCR Primer 1 (100% over 46bp)
GTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT201290.11091885323494402Illumina Single End PCR Primer 1 (100% over 46bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT199680.11003167874188297Illumina Single End PCR Primer 1 (100% over 46bp)
TTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC199540.10995453313379072Illumina Single End PCR Primer 1 (100% over 47bp)
GGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT199530.10994902273321269Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT197820.10900674423437146Illumina Single End PCR Primer 1 (100% over 46bp)
TACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC197420.10878632821125069Illumina Single End PCR Primer 1 (100% over 47bp)
AGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT194630.10724892644998339Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG194220.1070230000262846Illumina Single End PCR Primer 1 (100% over 45bp)
TCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG193620.10669237599160346Illumina Single End PCR Primer 1 (100% over 48bp)
ATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT191170.10534232784998883Illumina Single End PCR Primer 1 (100% over 46bp)
CCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG191070.10528722384420862Illumina Single End PCR Primer 1 (100% over 48bp)
AATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT188250.1037332908812073Illumina Single End PCR Primer 1 (100% over 46bp)
TATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC187990.1035900204661788Illumina Single End PCR Primer 1 (100% over 47bp)
GCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC186970.10302795960722086Illumina Single End PCR Primer 1 (100% over 47bp)
TGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC185480.10220690992109602Illumina Single End PCR Primer 1 (100% over 47bp)
CACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC182430.10052623774480024Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)