FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6A_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6A_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16905381
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2166871.281763481106992Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1598660.9456515650253609No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1420970.8405430200005549Illumina Single End PCR Primer 1 (100% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC899200.5319016471737609Illumina Single End PCR Primer 1 (100% over 49bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG561530.33216051149630993Illumina Single End PCR Primer 1 (100% over 48bp)
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC540410.31966744789721097Illumina Single End PCR Primer 1 (100% over 47bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC524070.3100018863816202Illumina Single End PCR Primer 1 (100% over 49bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC521330.30838110066847946Illumina Single End PCR Primer 1 (100% over 47bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG512040.302885808962247Illumina Single End PCR Primer 1 (100% over 48bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC472400.27943765360863504Illumina Single End PCR Primer 1 (100% over 47bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC471960.27917738145032045Illumina Single End PCR Primer 1 (100% over 49bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC435280.25748014788900647Illumina Single End PCR Primer 1 (100% over 47bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC392780.23234022350634986Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG366470.21677713149440406Illumina Single End PCR Primer 1 (100% over 48bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG357520.21148295918323284Illumina Single End PCR Primer 1 (100% over 48bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC310640.18375214377008126Illumina Single End PCR Primer 1 (100% over 47bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC305770.18087140419964506Illumina Single End PCR Primer 1 (100% over 47bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG293720.17374349622762125Illumina Single End PCR Primer 1 (100% over 48bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC293080.17336491854280006Illumina Single End PCR Primer 1 (100% over 47bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC292160.17282071312086963Illumina Single End PCR Primer 1 (100% over 47bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC286890.1697033624974202Illumina Single End PCR Primer 1 (100% over 47bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG284630.16836651004789538Illumina Single End PCR Primer 1 (100% over 48bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC283820.1678873726655436Illumina Single End PCR Primer 1 (98% over 50bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC277430.1641075110936571Illumina Single End PCR Primer 1 (100% over 47bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC272970.16146929785255948Illumina Single End PCR Primer 1 (100% over 47bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC260550.15412252465649842Illumina Single End PCR Primer 1 (100% over 47bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG258150.15270285833841898Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT257410.1522651278903445Illumina Single End PCR Primer 1 (100% over 46bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG253270.14981620349165747Illumina Single End PCR Primer 1 (100% over 48bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG248680.14710109165833055Illumina Single End PCR Primer 1 (100% over 48bp)
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC245120.14499525328651275Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG238300.140961034832637Illumina Single End PCR Primer 1 (100% over 48bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT235570.13934616439582165Illumina Single End PCR Primer 1 (100% over 46bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG221940.13128364276439555Illumina Single End PCR Primer 1 (100% over 48bp)
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT219850.13004735001240136Illumina Single End PCR Primer 1 (100% over 46bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC217450.12862768369432195Illumina Single End PCR Primer 1 (100% over 47bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC216590.1281189699303435Illumina Single End PCR Primer 1 (100% over 47bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC215570.1275156117451597Illumina Single End PCR Primer 1 (100% over 47bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT214690.1269950674285306Illumina Single End PCR Primer 1 (100% over 46bp)
GATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC214240.1267288799938907Illumina Single End PCR Primer 1 (100% over 47bp)
AGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT214040.12661057446738408Illumina Single End PCR Primer 1 (100% over 46bp)
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC211040.1248359915697848Illumina Single End PCR Primer 1 (100% over 47bp)
ACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC209060.12366476685736925Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC204300.12084909532651172Illumina Single End PCR Primer 1 (100% over 38bp)
GTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC203040.12010377050952002Illumina Single End PCR Primer 1 (100% over 40bp)
GTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA199250.11786188078221958Illumina Single End PCR Primer 1 (100% over 36bp)
GAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT198830.11761343917655567Illumina Single End PCR Primer 1 (100% over 46bp)
ATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC194140.11483917457997546Illumina Single End PCR Primer 1 (100% over 47bp)
TAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC189980.11237841962863776Illumina Single End PCR Primer 1 (100% over 47bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC184340.1090422037811511Illumina Single End PCR Primer 1 (100% over 47bp)
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG182650.10804252208217018Illumina Single End PCR Primer 1 (100% over 48bp)
GTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG179740.10632117667149885Illumina Single End PCR Primer 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)