FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6A_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6A_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5380857
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG644811.1983407104109995No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC572051.0631206144300063Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC429800.7987575213390729Illumina Single End PCR Primer 1 (100% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC280250.5208278160895188Illumina Single End PCR Primer 1 (100% over 49bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG145940.27122073677111286Illumina Single End PCR Primer 1 (100% over 48bp)
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC141830.26358254828180716Illumina Single End PCR Primer 1 (100% over 47bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC137120.2548292957794641Illumina Single End PCR Primer 1 (100% over 47bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC136060.2528593493564315Illumina Single End PCR Primer 1 (100% over 49bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG135570.25194871374578437Illumina Single End PCR Primer 1 (100% over 48bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC132470.24618754967842485Illumina Single End PCR Primer 1 (100% over 47bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC120660.22423937302180674Illumina Single End PCR Primer 1 (100% over 49bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC111890.20794085403124446Illumina Single End PCR Primer 1 (100% over 47bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC95250.1770164120696759Illumina Single End PCR Primer 1 (100% over 47bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG90730.16861626317146136Illumina Single End PCR Primer 1 (100% over 48bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG86580.1609037370812865Illumina Single End PCR Primer 1 (100% over 48bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC83690.15553284541849002Illumina Single End PCR Primer 1 (100% over 47bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC81930.1522619909802472Illumina Single End PCR Primer 1 (100% over 47bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC77000.14309988167312382Illumina Single End PCR Primer 1 (100% over 47bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG76780.14269102486834348Illumina Single End PCR Primer 1 (100% over 48bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC76050.1413343636524814Illumina Single End PCR Primer 1 (100% over 47bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC75950.1411485196503085Illumina Single End PCR Primer 1 (100% over 47bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG73360.1363351599940307Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT71990.13378909716426213Illumina Single End PCR Primer 1 (100% over 46bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC70260.13057399592667115Illumina Single End PCR Primer 1 (100% over 47bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC68040.12644825907843305Illumina Single End PCR Primer 1 (100% over 47bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT66000.12265704143410612Illumina Single End PCR Primer 1 (100% over 46bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC64350.11959061539825347Illumina Single End PCR Primer 1 (98% over 50bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC64320.1195348621976016Illumina Single End PCR Primer 1 (100% over 47bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG64120.11916317419325584Illumina Single End PCR Primer 1 (100% over 48bp)
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC62060.11533478774849433Illumina Single End PCR Primer 1 (100% over 47bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG61220.11377369813024207Illumina Single End PCR Primer 1 (100% over 48bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC60530.11249137451524915Illumina Single End PCR Primer 1 (100% over 47bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG59610.11078160969525859Illumina Single End PCR Primer 1 (100% over 48bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG59100.10983380528417684Illumina Single End PCR Primer 1 (100% over 48bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG57730.1072877424544083Illumina Single End PCR Primer 1 (100% over 48bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT57560.10697180765071439Illumina Single End PCR Primer 1 (100% over 46bp)
GTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA57310.10650719764528215Illumina Single End PCR Primer 1 (100% over 36bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC57020.10596825003898079Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT56710.10539213363224481Illumina Single End PCR Primer 1 (100% over 46bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC56710.10539213363224481Illumina Single End PCR Primer 1 (100% over 47bp)
GAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT56010.10409122561703461Illumina Single End PCR Primer 1 (100% over 46bp)
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC55380.10292040840334542Illumina Single End PCR Primer 1 (100% over 47bp)
AGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT55090.10238146079704404Illumina Single End PCR Primer 1 (100% over 46bp)
GTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC54010.10037434557357684Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)