FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6A_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6A_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870751
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC835401.4229865991591195Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC609151.0376014925518047Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373380.6360004026742064No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC300100.5111782121231168Illumina Single End PCR Primer 1 (100% over 49bp)
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGG143950.245198612579549No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC115930.1974704769457945Illumina Single End PCR Primer 1 (100% over 49bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG95490.16265380698312704Illumina Single End PCR Primer 1 (100% over 48bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC94930.1616999256142868Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC93050.1584976095903233Illumina Single End PCR Primer 1 (98% over 50bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG83430.14211129036131834Illumina Single End PCR Primer 1 (100% over 48bp)
AAAGGTTGTTTTATAGTAGTTAGGATGTAGAAAATGTATTCCGATAGGCC74740.12730909554842304No Hit
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC73110.12453261942126313Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC70130.11945660785136349Illumina Single End PCR Primer 1 (100% over 40bp)
GGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC61710.10511432012701612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)