FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839674
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA112840.397369557209736TruSeq Adapter, Index 27 (97% over 44bp)
CTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT72810.25640267157427227TruSeq Adapter, Index 27 (100% over 39bp)
CTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC70380.24784535126215193TruSeq Adapter, Index 13 (97% over 37bp)
ACACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA52610.18526774552290157Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGT47700.1679770283490288TruSeq Adapter, Index 27 (97% over 44bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGT41090.14469970848766445TruSeq Adapter, Index 27 (97% over 44bp)
CACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT36490.12850066592151071TruSeq Adapter, Index 1 (100% over 35bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCG28790.10138487727816643TruSeq Adapter, Index 27 (97% over 44bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT28490.1003284179803738TruSeq Adapter, Index 27 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)