FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054859
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA158650.5193365716715568TruSeq Adapter, Index 25 (97% over 44bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGT61200.20033657854585107TruSeq Adapter, Index 25 (97% over 44bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGT50880.16655433196753108TruSeq Adapter, Index 25 (97% over 44bp)
CTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGAT44470.14557136679630714TruSeq Adapter, Index 25 (100% over 39bp)
CTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG40210.13162636966223318TruSeq Adapter, Index 5 (97% over 37bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTC34390.11257475385934343TruSeq Adapter, Index 25 (97% over 44bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTC34250.11211646756855227TruSeq Adapter, Index 25 (97% over 44bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCG32570.1066170320790583TruSeq Adapter, Index 25 (97% over 44bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTC31500.10311441542801157TruSeq Adapter, Index 25 (97% over 44bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCG31380.10272159860733343TruSeq Adapter, Index 25 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)