FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7681443
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1182581.5395284453715272TruSeq Adapter, Index 9 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT594390.7737999227488898TruSeq Adapter, Index 9 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT326770.4254018418153985TruSeq Adapter, Index 9 (100% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT140090.18237458769140122TruSeq Adapter, Index 9 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATT117310.15271870142107416TruSeq Adapter, Index 9 (97% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT102710.13371185596247998TruSeq Adapter, Index 9 (100% over 48bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTC100420.13073064527068676Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACT99420.1294288065406461Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA93690.12196927061751289TruSeq Adapter, Index 9 (100% over 47bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA88580.11531687470700493TruSeq Adapter, Index 9 (100% over 47bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAG88060.11463991856738376Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA85150.11085156786296534Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAAC83500.10870353395839819Illumina Single End PCR Primer 1 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)