FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12421634
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTT194760.15679096647027274TruSeq Adapter, Index 8 (97% over 42bp)
CTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA178510.14370895165644068TruSeq Adapter, Index 8 (100% over 39bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAG166420.13397593263494964Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA144590.116401755195814Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTT143920.1158623736619514TruSeq Adapter, Index 8 (97% over 42bp)
CTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT136870.11018679185041194TruSeq Adapter, Index 8 (100% over 37bp)
CTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT125900.10135542554224347Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)