FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3614618
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT47550.13154917061775268TruSeq Adapter, Index 10 (100% over 46bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT46880.12969558608959508TruSeq Adapter, Index 10 (100% over 46bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT43930.12153428107755786TruSeq Adapter, Index 10 (100% over 46bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT37670.10421571518760765TruSeq Adapter, Index 10 (100% over 46bp)
TTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT36170.10006589907979212TruSeq Adapter, Index 10 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)