FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9789118
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATT207820.2122969607680692TruSeq Adapter, Index 7 (97% over 48bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT144510.14762310557498642TruSeq Adapter, Index 7 (100% over 46bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT139770.1427809941610674TruSeq Adapter, Index 7 (100% over 46bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT121670.12429107504884505TruSeq Adapter, Index 7 (100% over 46bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG118730.1212877401212244TruSeq Adapter, Index 7 (100% over 45bp)
GCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT115770.11826397434375599TruSeq Adapter, Index 7 (100% over 46bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC112280.11469879104532196TruSeq Adapter, Index 7 (100% over 44bp)
CCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC102340.10454465867098547TruSeq Adapter, Index 7 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)