FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20028199
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT1133620.5660119514490544TruSeq Adapter, Index 6 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1007720.5031505828357308TruSeq Adapter, Index 6 (100% over 49bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA898610.44867239435757555TruSeq Adapter, Index 6 (100% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATT830110.4144706171533446TruSeq Adapter, Index 6 (97% over 48bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT808590.4037257668550228TruSeq Adapter, Index 6 (100% over 46bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA806410.40263730153669836TruSeq Adapter, Index 6 (100% over 47bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT804700.4017835053466365TruSeq Adapter, Index 6 (100% over 46bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT781120.3900101052520998TruSeq Adapter, Index 6 (100% over 48bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT768340.38362910214742724TruSeq Adapter, Index 6 (100% over 46bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT722570.36077632342279004TruSeq Adapter, Index 6 (100% over 48bp)
TTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA679590.33931658058720104TruSeq Adapter, Index 6 (100% over 47bp)
TTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT673840.336445628486116TruSeq Adapter, Index 6 (100% over 46bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT653520.32629993340889013TruSeq Adapter, Index 6 (100% over 46bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA582910.29104464160756544TruSeq Adapter, Index 6 (100% over 47bp)
GCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT537700.26847146865277305TruSeq Adapter, Index 6 (100% over 46bp)
TCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT523290.26127661303944505TruSeq Adapter, Index 6 (100% over 46bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA516460.25786642123937353TruSeq Adapter, Index 6 (100% over 47bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT509630.2544562294393021TruSeq Adapter, Index 6 (100% over 48bp)
GTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT498270.2487842266795931TruSeq Adapter, Index 6 (100% over 46bp)
TGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA484050.24168423730960534TruSeq Adapter, Index 6 (100% over 47bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG483740.24152945554415553TruSeq Adapter, Index 6 (100% over 45bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA481610.2404659550267101TruSeq Adapter, Index 6 (100% over 47bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA481280.2403011873409087TruSeq Adapter, Index 6 (100% over 47bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT478910.23911785577924405TruSeq Adapter, Index 6 (100% over 46bp)
GCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT464550.23194796496679507TruSeq Adapter, Index 6 (100% over 46bp)
ACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT458080.22871751973305238TruSeq Adapter, Index 6 (100% over 46bp)
CATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT455070.22721463872013656TruSeq Adapter, Index 6 (100% over 46bp)
TAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA442920.2211481921065394TruSeq Adapter, Index 6 (100% over 47bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT418340.20887549599442265TruSeq Adapter, Index 6 (100% over 46bp)
CCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG398730.19908430108967862TruSeq Adapter, Index 6 (100% over 45bp)
ATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT398700.19906932220915122TruSeq Adapter, Index 6 (100% over 46bp)
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT393910.19667769428494294TruSeq Adapter, Index 6 (100% over 46bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA390710.1950799470286869TruSeq Adapter, Index 6 (100% over 47bp)
CTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT382610.1910356492862888TruSeq Adapter, Index 6 (100% over 46bp)
CCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT379470.18946785979108757TruSeq Adapter, Index 6 (100% over 46bp)
TGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT371740.18560830157519403TruSeq Adapter, Index 6 (100% over 46bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA363540.18151407423103794TruSeq Adapter, Index 6 (100% over 47bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT362860.18117455293908355TruSeq Adapter, Index 6 (100% over 46bp)
CCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG362780.18113460925767716TruSeq Adapter, Index 6 (100% over 45bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT358710.17910247446612648TruSeq Adapter, Index 6 (100% over 46bp)
CCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG358510.1790026152626105TruSeq Adapter, Index 6 (100% over 45bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC351810.17565733194482439TruSeq Adapter, Index 6 (100% over 44bp)
ATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT333260.16639539081871516TruSeq Adapter, Index 6 (100% over 46bp)
TATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT332280.16590608072148674TruSeq Adapter, Index 6 (100% over 46bp)
TTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT329910.1647227491598221TruSeq Adapter, Index 6 (100% over 46bp)
CTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG326000.16277050173108426TruSeq Adapter, Index 6 (100% over 45bp)
CTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG300780.15017825616771632TruSeq Adapter, Index 6 (100% over 45bp)
ATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA297290.1484357130663621TruSeq Adapter, Index 6 (100% over 47bp)
CCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC288670.14413178139482236TruSeq Adapter, Index 6 (100% over 44bp)
GCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG280830.14021730061699506TruSeq Adapter, Index 6 (100% over 45bp)
GCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG274480.137046770905362TruSeq Adapter, Index 6 (100% over 45bp)
TTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT259250.1294424925576184TruSeq Adapter, Index 6 (100% over 46bp)
GTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG258710.12917287270812516TruSeq Adapter, Index 6 (100% over 45bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG251540.12559292026207647TruSeq Adapter, Index 6 (100% over 45bp)
GCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG240010.11983603717937895TruSeq Adapter, Index 6 (100% over 45bp)
GATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT237340.11850291681244031TruSeq Adapter, Index 6 (100% over 46bp)
CCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC235960.11781388830817989TruSeq Adapter, Index 6 (100% over 44bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT233900.11678533851196506TruSeq Adapter, Index 6 (100% over 46bp)
TCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT232240.11595650712278223TruSeq Adapter, Index 6 (100% over 46bp)
CCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG231160.11541726742379582TruSeq Adapter, Index 6 (100% over 45bp)
CTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT225760.11272106892886374TruSeq Adapter, Index 6 (100% over 46bp)
CCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG225420.11255130828288654TruSeq Adapter, Index 6 (100% over 45bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT223530.11160763880966032TruSeq Adapter, Index 6 (100% over 46bp)
GTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG222260.11097353286733369TruSeq Adapter, Index 6 (100% over 45bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT221670.11067894821696149TruSeq Adapter, Index 6 (100% over 46bp)
CACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG218720.10920602496510044TruSeq Adapter, Index 6 (100% over 45bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG213930.1068143970408922TruSeq Adapter, Index 6 (100% over 45bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT211220.10546130483325036TruSeq Adapter, Index 6 (100% over 46bp)
CTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG211150.10542635411201977TruSeq Adapter, Index 6 (100% over 45bp)
GCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT210620.10516172722270237TruSeq Adapter, Index 6 (100% over 46bp)
CCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG209100.10440279727598073TruSeq Adapter, Index 6 (100% over 45bp)
TTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT205940.10282502186042788TruSeq Adapter, Index 6 (100% over 46bp)
CACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG205230.10247052168794608TruSeq Adapter, Index 6 (100% over 45bp)
CAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT204700.10220589479862867TruSeq Adapter, Index 6 (100% over 46bp)
AACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT202230.10097263363520605TruSeq Adapter, Index 6 (100% over 46bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC201430.10057319682114203TruSeq Adapter, Index 6 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)