FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15076549
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATT774280.5135658034209287TruSeq Adapter, Index 5 (97% over 48bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTAT625480.4148694770931995TruSeq Adapter, Index 5 (97% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTAT407340.27018119332215884TruSeq Adapter, Index 5 (97% over 48bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT339560.2252239554290574TruSeq Adapter, Index 5 (97% over 46bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT335290.22239174230123884TruSeq Adapter, Index 5 (97% over 46bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT323410.21451195495733139TruSeq Adapter, Index 5 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATT308700.20475508022426087TruSeq Adapter, Index 5 (97% over 41bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTA295530.1960196594061413TruSeq Adapter, Index 5 (97% over 47bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTA278960.18502908059397413TruSeq Adapter, Index 5 (97% over 47bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT266750.1769304102682915TruSeq Adapter, Index 5 (97% over 46bp)
TTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT259320.1720022267695346TruSeq Adapter, Index 5 (97% over 46bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTAT237540.157555949972371TruSeq Adapter, Index 5 (97% over 48bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAG208580.1383473101171893Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT204950.13593959731766203TruSeq Adapter, Index 5 (97% over 46bp)
GTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT204030.13532937809574325TruSeq Adapter, Index 5 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATT201500.1336512752354667TruSeq Adapter, Index 5 (97% over 48bp)
TCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT197050.13069967139031619TruSeq Adapter, Index 5 (97% over 46bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTAT196290.13019557725047026TruSeq Adapter, Index 5 (97% over 48bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTA190360.1262623163961461TruSeq Adapter, Index 5 (97% over 47bp)
TTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTA190210.12616282413170282TruSeq Adapter, Index 5 (97% over 47bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT183160.12148668770286888TruSeq Adapter, Index 5 (97% over 46bp)
ACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT179820.1192713266145986TruSeq Adapter, Index 5 (97% over 46bp)
CATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT178900.11866110739267985TruSeq Adapter, Index 5 (97% over 46bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT173080.11480080753228078TruSeq Adapter, Index 5 (97% over 46bp)
CTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA172500.11441610410976677Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT164720.10925577199397554TruSeq Adapter, Index 5 (97% over 46bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCG163970.10875831067175916TruSeq Adapter, Index 5 (97% over 45bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTA161840.10734552051666466TruSeq Adapter, Index 5 (97% over 47bp)
CTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT157910.10473882318825084TruSeq Adapter, Index 5 (97% over 46bp)
CTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT157360.1043740182186255Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT153850.10204589923065285TruSeq Adapter, Index 5 (97% over 46bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACT153630.10189997724280271Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTA153250.10164793017287976TruSeq Adapter, Index 5 (97% over 47bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT153190.10160813326710244TruSeq Adapter, Index 5 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)