FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18147501
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA1593120.8778729368853596TruSeq Adapter, Index 14 (97% over 44bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGT916320.5049290257650352TruSeq Adapter, Index 14 (97% over 44bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG868710.4786940086130867TruSeq Adapter, Index 14 (97% over 44bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC832400.4586857441142998TruSeq Adapter, Index 14 (97% over 44bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC819540.4515993689709674TruSeq Adapter, Index 14 (97% over 44bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGT810800.4467832788657788TruSeq Adapter, Index 14 (97% over 44bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG784780.4324452165617734TruSeq Adapter, Index 14 (97% over 44bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC784540.432312966947901TruSeq Adapter, Index 14 (97% over 44bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGT765470.4218046330456188TruSeq Adapter, Index 14 (97% over 44bp)
TTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC704140.3880093463006284TruSeq Adapter, Index 14 (97% over 44bp)
TTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG692910.38182116645151304TruSeq Adapter, Index 14 (97% over 44bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC674080.37144508216310335TruSeq Adapter, Index 14 (97% over 44bp)
TCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC561740.3095412420696381TruSeq Adapter, Index 14 (97% over 44bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG558590.30780546588756214TruSeq Adapter, Index 14 (97% over 44bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC515370.2839895145893641TruSeq Adapter, Index 14 (97% over 44bp)
GTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC508640.28028101500035735TruSeq Adapter, Index 14 (97% over 44bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG507440.27961976693099505TruSeq Adapter, Index 14 (97% over 44bp)
GCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC502220.27674333782926913TruSeq Adapter, Index 14 (97% over 44bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGT497670.27423610556627054TruSeq Adapter, Index 14 (97% over 44bp)
ACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC493680.272037455735641TruSeq Adapter, Index 14 (97% over 44bp)
CATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC474370.26139687221948626TruSeq Adapter, Index 14 (97% over 44bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT468780.2583165582963737TruSeq Adapter, Index 14 (97% over 44bp)
TGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG454950.2506956742969735TruSeq Adapter, Index 14 (97% over 44bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG454220.25029341505477803TruSeq Adapter, Index 14 (97% over 44bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC449130.24748862116056639TruSeq Adapter, Index 14 (97% over 44bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG447570.24662899867039545TruSeq Adapter, Index 14 (97% over 44bp)
TAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG435440.23994488276925843TruSeq Adapter, Index 14 (97% over 44bp)
GCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC430890.23743765050625978TruSeq Adapter, Index 14 (97% over 44bp)
ATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC426520.23502960545366552TruSeq Adapter, Index 14 (97% over 44bp)
TGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC408490.2250943532114973TruSeq Adapter, Index 14 (97% over 44bp)
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC402540.221815664867576TruSeq Adapter, Index 14 (97% over 44bp)
CCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT395750.2180741028751011TruSeq Adapter, Index 14 (97% over 44bp)
CTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC391780.21588647384562756TruSeq Adapter, Index 14 (97% over 44bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG387080.21329658557395864TruSeq Adapter, Index 14 (97% over 44bp)
CCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT384010.21160489259650683TruSeq Adapter, Index 14 (97% over 44bp)
ATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC374550.20639205364970087TruSeq Adapter, Index 14 (97% over 44bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC370910.20438626783930192TruSeq Adapter, Index 14 (97% over 44bp)
CCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC367110.20229231561965472TruSeq Adapter, Index 14 (97% over 44bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC366010.20168617155607266TruSeq Adapter, Index 14 (97% over 44bp)
TATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC361780.19935527211157061TruSeq Adapter, Index 14 (97% over 44bp)
CCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT358800.19771317273932096TruSeq Adapter, Index 14 (97% over 44bp)
TTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC351830.19387242353644174TruSeq Adapter, Index 14 (97% over 44bp)
CTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT350650.1932221962682355TruSeq Adapter, Index 14 (97% over 44bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG330480.1821077183023712TruSeq Adapter, Index 14 (97% over 44bp)
CTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT330070.18188179187867243TruSeq Adapter, Index 14 (97% over 44bp)
ATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCG301150.16594571340704156TruSeq Adapter, Index 14 (97% over 44bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATC281400.15506267226545406TruSeq Adapter, Index 14 (97% over 44bp)
GTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT269640.14858244118570377TruSeq Adapter, Index 14 (97% over 44bp)
TTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC267820.1475795482805043TruSeq Adapter, Index 14 (97% over 44bp)
GCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT262600.1447031191787784TruSeq Adapter, Index 14 (97% over 44bp)
GATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC244780.13488358534874856TruSeq Adapter, Index 14 (97% over 44bp)
GCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT243840.13436560769441477TruSeq Adapter, Index 14 (97% over 44bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC241030.13281718513199145TruSeq Adapter, Index 14 (97% over 44bp)
TCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC239710.13208981225569294TruSeq Adapter, Index 14 (97% over 44bp)
AACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC237710.13098773214008916TruSeq Adapter, Index 14 (97% over 44bp)
TTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC237090.13064608730425198TruSeq Adapter, Index 14 (97% over 44bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC234980.12948339278228996TruSeq Adapter, Index 14 (97% over 44bp)
GTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT231200.1274004613637988TruSeq Adapter, Index 14 (97% over 44bp)
CTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT228780.1260669444239182TruSeq Adapter, Index 14 (97% over 44bp)
CCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT222720.1227276416736387TruSeq Adapter, Index 14 (97% over 44bp)
CCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT222510.1226119232615003TruSeq Adapter, Index 14 (97% over 44bp)
CTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC221980.1223198720308653TruSeq Adapter, Index 14 (97% over 44bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT221430.12201679999907425TruSeq Adapter, Index 14 (97% over 44bp)
GCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT217960.12010469099850167TruSeq Adapter, Index 14 (97% over 44bp)
TACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC216510.11930568291468892TruSeq Adapter, Index 14 (97% over 44bp)
CCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATC215310.11864443484532664TruSeq Adapter, Index 14 (97% over 44bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC215070.11851218523145418TruSeq Adapter, Index 14 (97% over 44bp)
CACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT214720.11831932121122353TruSeq Adapter, Index 14 (97% over 44bp)
AATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC213460.11762501073839311TruSeq Adapter, Index 14 (97% over 44bp)
CCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT211700.11665518023666178TruSeq Adapter, Index 14 (97% over 44bp)
CACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT211700.11665518023666178TruSeq Adapter, Index 14 (97% over 44bp)
CATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT206830.11397161515516653TruSeq Adapter, Index 14 (97% over 44bp)
CAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC204580.11273177502511228TruSeq Adapter, Index 14 (97% over 44bp)
AGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC203870.11234053658407293TruSeq Adapter, Index 14 (97% over 44bp)
ACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC201180.11085823882858582TruSeq Adapter, Index 14 (97% over 44bp)
CCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT200690.1105882292002629TruSeq Adapter, Index 14 (97% over 44bp)
TGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC197050.10858244338986399TruSeq Adapter, Index 14 (97% over 44bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT194400.10712218723668895TruSeq Adapter, Index 14 (97% over 44bp)
CCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATC191560.10555723347253157TruSeq Adapter, Index 14 (97% over 44bp)
GTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC191230.10537539025345694TruSeq Adapter, Index 14 (97% over 44bp)
CTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT189070.10418514372860484TruSeq Adapter, Index 14 (97% over 44bp)
CGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT187470.1033034796361218TruSeq Adapter, Index 14 (97% over 44bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC187020.10305551161011095TruSeq Adapter, Index 14 (97% over 44bp)
CATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCT186720.10289019959277039TruSeq Adapter, Index 14 (97% over 44bp)
GCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTC182860.10076318496965504TruSeq Adapter, Index 14 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)