FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16905381
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTA2765851.6360767024416665TruSeq Adapter, Index 13 (97% over 39bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT1679660.9935653032605417TruSeq Adapter, Index 13 (97% over 39bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT1038020.6140175131220054TruSeq Adapter, Index 13 (97% over 39bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG636370.3764304395150869TruSeq Adapter, Index 13 (97% over 39bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG624860.369621956464631TruSeq Adapter, Index 13 (97% over 39bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC593910.35131417623773165TruSeq Adapter, Index 13 (97% over 39bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT593600.3511308026716464TruSeq Adapter, Index 13 (97% over 39bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC588560.34814950340367956TruSeq Adapter, Index 13 (97% over 39bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC574620.3399036082061682TruSeq Adapter, Index 13 (97% over 39bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT533670.3156805516539379TruSeq Adapter, Index 13 (97% over 39bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC485420.28713934338421593TruSeq Adapter, Index 13 (97% over 39bp)
TTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC427640.2529608767764536TruSeq Adapter, Index 13 (97% over 39bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG413480.24458484549978493TruSeq Adapter, Index 13 (97% over 39bp)
TTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG403440.23864590806915265TruSeq Adapter, Index 13 (97% over 39bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC356060.21061932883973453TruSeq Adapter, Index 13 (97% over 39bp)
TCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC343570.20323114870939613TruSeq Adapter, Index 13 (97% over 39bp)
ACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC326190.19295039845597092TruSeq Adapter, Index 13 (97% over 39bp)
GTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC316310.1871061054465439TruSeq Adapter, Index 13 (97% over 39bp)
GCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC314870.18625430565569626TruSeq Adapter, Index 13 (97% over 39bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG313450.18541433641749927TruSeq Adapter, Index 13 (97% over 39bp)
CATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC313190.18526053923304064TruSeq Adapter, Index 13 (97% over 39bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC306630.18138011796362355TruSeq Adapter, Index 13 (97% over 39bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG304420.1800728418957254TruSeq Adapter, Index 13 (97% over 39bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCT299930.1774168828256518TruSeq Adapter, Index 13 (97% over 39bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAG292310.17290944226574959Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG285840.16908225848326044TruSeq Adapter, Index 13 (97% over 39bp)
TGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG285790.1690526821016338TruSeq Adapter, Index 13 (97% over 39bp)
ATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC283860.16791103377084493TruSeq Adapter, Index 13 (97% over 39bp)
TAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG274740.16251630176214307TruSeq Adapter, Index 13 (97% over 39bp)
CTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC274450.16234475874870846TruSeq Adapter, Index 13 (97% over 39bp)
CCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCT269460.1593930358623683TruSeq Adapter, Index 13 (97% over 39bp)
CTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA261890.15491517168409277TruSeq Adapter, Index 13 (100% over 39bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG259260.15335945401053075TruSeq Adapter, Index 13 (97% over 39bp)
CCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC256340.1516321933235341TruSeq Adapter, Index 13 (97% over 39bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG247730.14653914040742413TruSeq Adapter, Index 13 (97% over 39bp)
CTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCT244530.1446462519833182TruSeq Adapter, Index 13 (97% over 39bp)
GCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC240970.14254041361150038TruSeq Adapter, Index 13 (97% over 39bp)
CTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCT240390.1421973275846312TruSeq Adapter, Index 13 (97% over 39bp)
CCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCT239590.1417241054786047TruSeq Adapter, Index 13 (97% over 39bp)
CTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC237080.14023937112094664TruSeq Adapter, Index 13 (100% over 37bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC236780.1400619128311867TruSeq Adapter, Index 13 (97% over 39bp)
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC234360.1386304159604566TruSeq Adapter, Index 13 (97% over 39bp)
ATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC234340.13861858540780594TruSeq Adapter, Index 13 (97% over 39bp)
CACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG233300.13800339666997155TruSeq Adapter, Index 2 (97% over 35bp)
TGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC231040.13666654422044672TruSeq Adapter, Index 13 (97% over 39bp)
CTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA230080.13609867769321496Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCT224550.13282752988530694TruSeq Adapter, Index 13 (97% over 39bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCC222060.13135462608029952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC213650.12637987869069617TruSeq Adapter, Index 13 (97% over 39bp)
CTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT212100.12546301086026987Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC209680.1240315139895398TruSeq Adapter, Index 13 (97% over 39bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTC207610.12280705179019628TruSeq Adapter, Index 13 (97% over 39bp)
ACACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA207210.12257044073718303Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCG198180.11722894621540916TruSeq Adapter, Index 13 (97% over 39bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTC196050.11596899235811367Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACT195500.11564365216022046Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCGTAGATCGGAAGAGCACACGTCTGAAC183760.1086991177542819Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC182730.10808984429277282TruSeq Adapter, Index 13 (100% over 37bp)
CTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATA180290.10664651686939206TruSeq Adapter, Index 13 (97% over 39bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT179590.1062324475266189Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATC177220.10483052703751546TruSeq Adapter, Index 13 (97% over 39bp)
CTCTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGT173620.10270102756039631Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT172530.10205626244093521TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)