FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5380857
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT176430.32788457303362645TruSeq Adapter, Index 12 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATT171270.3182950225215054TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATT156650.29112462940382916TruSeq Adapter, Index 12 (97% over 48bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTAT147660.2744172536084865TruSeq Adapter, Index 12 (97% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT109450.203406260378226TruSeq Adapter, Index 12 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG97000.18026868210770142TruSeq Adapter, Index 12 (100% over 49bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTAT96620.17956247489944446TruSeq Adapter, Index 12 (97% over 48bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA83690.15553284541849002TruSeq Adapter, Index 12 (100% over 47bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT82960.15417618420262794TruSeq Adapter, Index 12 (100% over 46bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT82140.15265226338481028TruSeq Adapter, Index 12 (100% over 46bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA82070.15252217258328926TruSeq Adapter, Index 12 (100% over 47bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT79820.14834068253439928TruSeq Adapter, Index 12 (100% over 46bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAG73490.13657675719685544Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA67810.1260208178734354TruSeq Adapter, Index 12 (100% over 39bp)
CACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT66560.1236977678462743TruSeq Adapter, Index 1 (97% over 35bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT65440.12161631502193795TruSeq Adapter, Index 12 (100% over 46bp)
CTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG62300.11578081335370927TruSeq Adapter, Index 12 (100% over 37bp)
CTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA61690.11464716494045464Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC60220.1119152581085132Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCC58920.10949928608026566Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACT55880.10384962841420986Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)