FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n01_dad6A_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n01_dad6A_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870751
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG806861.3743727165400133TruSeq Adapter, Index 11 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT793081.3509004214281954TruSeq Adapter, Index 11 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT383840.6538175439564716TruSeq Adapter, Index 11 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATT374830.6384702740756676TruSeq Adapter, Index 11 (97% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT144400.2459651243937956TruSeq Adapter, Index 11 (100% over 48bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA126270.215083215077594TruSeq Adapter, Index 11 (100% over 47bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT121400.20678785388785864TruSeq Adapter, Index 11 (100% over 48bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAG113620.19353571629932867Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA113330.1930417420190364TruSeq Adapter, Index 11 (100% over 47bp)
CTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC113020.19251370054699987TruSeq Adapter, Index 11 (100% over 39bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTC109840.18709701705965726Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACT109410.18636457243715496Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA107630.1833325923719129Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT90230.15369413555437797Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT89110.15178637281669757TruSeq Adapter, Index 11 (100% over 46bp)
CTTTCCCTACACGACGCTCTTCCGTAGATCGGAAGAGCACACGTCTGAAC86270.14694883158900796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAAC83880.14287780217556492Illumina Single End PCR Primer 1 (96% over 25bp)
CTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT79590.13557038954641407TruSeq Adapter, Index 11 (100% over 41bp)
CTTTCCCTACACGACGCTCTTCCTCAGATCGGAAGAGCACACGTCTGAAC76950.1310735202361674Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCC75420.12846738006772898Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT71750.12221605038265121TruSeq Adapter, Index 11 (100% over 46bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA69730.11877526401647763Illumina Single End PCR Primer 1 (96% over 26bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT69690.11870712963298903TruSeq Adapter, Index 11 (100% over 46bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAAC68090.1159817542934456Illumina Single End PCR Primer 1 (96% over 25bp)
CTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT67840.11555591439664194TruSeq Adapter, Index 11 (100% over 37bp)
CTCTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGT66420.11313714378279713Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA66200.1127624046736099TruSeq Adapter, Index 11 (100% over 40bp)
CTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAA66120.11262613590663274Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCCA64290.10950898786202992Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCCTACACGACGCTCTTCCGCAGATCGGAAGAGCACACGTCTGAAC64110.10920238313633128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT64090.109168315944587TruSeq Adapter, Index 11 (100% over 46bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA63130.10753309074086091TruSeq Adapter, Index 11 (100% over 47bp)
CTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCA60250.10262741512968272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT60130.10242301197921698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCTTAGATCGGAAGAGCACACGTCTGAAC59700.10169056735671467Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC58740.1000553421529886TruSeq Adapter, Index 11 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)