FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32321854
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC658510.2037352189017375TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT84050.036.692942
TCGTATG84300.036.66219743
CGTATGC84800.036.44602644
TCTCGTA84600.036.4283541
ACGGCTA88800.034.69183732
CGGCTAC89400.034.1877233
ATCTCGT94700.032.47348840
TCACGGC99900.031.03609730
CATCTCG100800.030.46468239
GTCACGG102950.030.43723729
CGTCTGA104200.030.33720616
AGTCACG104300.029.89559228
ACACGTC107150.029.56301713
GATCGGA108350.029.1451361
ACGTCTG109450.029.08282715
ATCGGAA112700.028.5177232
CACGGCT110400.028.1040531
CACGTCT124000.025.86526914
TCGGAAG129050.025.09213
CGGAAGA130500.024.627914
Produced by FastQC (version 0.11.2)