FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31170444
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1325430.4252201219847879TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC158000.039.7622844
CTCGTAT158700.039.3930342
TCGTATG159500.039.3608243
TCTCGTA161300.038.78532841
TATCTCG163350.038.28517539
ATCTCGT168750.037.0730340
GCTTATC185250.034.0692636
TAGCTTA187800.033.89512634
CGTCTGA196400.032.75070616
GATCGGA196550.032.5382961
ATCGGAA202050.032.4123882
AGCTTAT198300.032.1198935
ACACGTC203300.031.72494713
ACGTCTG205050.031.43303715
TTATCTC204950.030.88975138
ACTAGCT208450.030.8874932
GTCACTA209250.030.88572129
CTTATCT211000.030.11901737
TCACTAG218300.029.6254130
CACGTCT219150.029.46074514
Produced by FastQC (version 0.11.2)