FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33428117
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4157981.2438570799545783TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC455800.042.07359344
TCGTATG477650.040.1259843
CTCGTAT476550.039.94182242
TCTCGTA476050.039.8821241
GATCTCG480750.039.36409839
CGATCAG487250.039.28981433
ATCTCGT485250.038.98998340
ACGATCA492950.038.95643632
GTCACGA497200.038.58867329
CACGATC498000.038.5619231
TCACGAT501250.038.30761330
ATCGGAA524250.038.021292
GATCGGA517200.038.0156481
AGTCACG512700.037.49456828
CGTCTGA523200.037.34015716
TCGGAAG535800.037.0820053
ACACGTC530550.037.0084513
ACGTCTG531050.036.8623715
CGGAAGA538900.036.7625054
CACGTCT547650.035.82905214
Produced by FastQC (version 0.11.2)