FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28658829
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1742850.6081371991856331TruSeq Adapter, Index 8 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288880.10079965235146209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC211150.039.42087644
TCGTATG210900.039.27986543
CTCGTAT209800.039.14008742
TCTCGTA212300.038.61701641
AATCTCG213550.038.4115739
ATCTCGT219650.037.35484340
TTGAATC249200.033.31517436
CGTCTGA267700.032.58194416
GAATCTC253900.032.52393738
ATCGGAA273600.032.289442
GATCGGA267950.032.0874181
TGAATCT259100.031.99854937
ACACGTC274750.031.8970813
ACGTCTG274400.031.88226115
CTTGAAT266550.031.50587835
CACGTCT285400.030.68411314
TCGGAAG296850.029.7380163
CGGAAGA298100.029.598564
CACACGT313300.028.11272812
GTCACAC311550.027.93509729
Produced by FastQC (version 0.11.2)