FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29542352
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC299660.10143403612549197TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38050.036.19003344
TCGTATG39950.034.5789843
TCTCGTA40050.034.05341341
CTCGTAT40450.033.9885842
ATCTCGT48300.028.51009840
CGTCTGA49600.028.47331816
CATCTCG49000.028.14770339
ACGTCTG53300.026.70260815
ACACGTC60300.023.52960413
GCACACG69500.020.69971811
CACACGT70150.020.44520212
CACGTCT71350.020.07068414
GATCGGA73700.019.0806451
ATCGGAA76200.018.7647952
CGGAAGA81800.017.5338024
CTTAGGC80700.017.53248633
TCGGAAG83100.017.4447783
TAGGCAT88300.015.84652635
AGGCATC91100.015.55091436
TTAGGCA90900.015.49199734
Produced by FastQC (version 0.11.2)