FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44367966
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC10695862.4107167770548688TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1164350.042.7534641
CGTATGC1164850.042.7180844
CGTCTGA1181400.042.61833216
ATCGGAA1192300.042.5058942
AATCTCG1159400.042.47908439
ACGTCTG1186700.042.4460515
CTCGTAT1162550.042.4376942
TCTCGTA1162450.042.40732241
TCGTATG1172950.042.40629243
ACACGTC1188400.042.3994213
TCGGAAG1201750.042.1238673
ATCTCGT1170400.042.11363240
CGGAAGA1203450.042.016744
CACGTCT1206300.041.7869614
GCACACG1207050.041.7605711
CACACGT1213750.041.5355312
GTAATCT1218900.040.38392337
AGTCACC1253450.039.9576628
GAACTCC1266300.039.6629721
TGTAATC1244550.039.5659136
Produced by FastQC (version 0.11.2)