FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35525976
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5452241.534719271329801TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC589500.042.85300444
CTCGTAT587800.042.5990942
TCTCGTA587100.042.5487241
TCGTATG591550.042.5149143
CGTCTGA602400.042.30935316
ACGTCTG606700.042.05965415
ACACGTC610500.041.85858513
CATCTCG596050.041.82125539
ATCTCGT597800.041.79450240
GATCGGA603500.041.7776571
ATCGGAA620050.041.38512
CGGAAGA623300.041.059624
CACGTCT622450.041.04129814
TCGGAAG625050.041.0255473
GCACACG626800.040.80848311
CACACGT629000.040.65525412
GATCATC630650.039.7133736
AGTCACC658550.038.55637728
CCAGATC663600.038.1227333
GAACTCC669200.038.10010521
Produced by FastQC (version 0.11.2)