FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37112943
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4128611.1124447877927655TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC452500.042.58465644
GATCGGA469800.041.771551
TCGTATG465650.041.34432243
CGTCTGA472500.041.32367316
TCTCGTA460800.041.31176841
CGATCTC457000.041.28957438
CACGATC456600.041.27491836
CTCGTAT463250.041.24523542
ATCGGAA482400.041.2427672
GATCTCG458200.041.23893739
ACGATCT458200.041.13364837
ACACGTC478200.040.9358113
ACGTCTG477800.040.9106115
TCACGAT465100.040.86252235
CATCACG473250.040.5627433
ATCACGA470100.040.5573934
TCGGAAG490900.040.4652523
ATCTCGT468200.040.42861640
CGGAAGA493750.040.1827244
CACGTCT491850.039.83131814
Produced by FastQC (version 0.11.2)