FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34088919
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC8936172.621429561905439TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC946900.042.9977144
GATCGGA947550.042.9861341
CGCCAAT946550.042.81613533
ATCGGAA964800.042.7651332
CGTCTGA959700.042.7621716
GTCACGC950550.042.73976529
ACGTCTG961750.042.69110515
TCGTATG954250.042.66210643
TCTCGTA952200.042.65461341
CTCGTAT952050.042.6451642
ACGCCAA952900.042.60109332
ACACGTC965550.042.59555413
TATCTCG954850.042.5201139
TCGGAAG971800.042.454583
CGGAAGA973100.042.386624
TCACGCC959400.042.3340430
AGTCACG962450.042.29535728
CACGCCA960550.042.28106731
ATCTCGT961750.042.23792640
GCACACG978450.042.0452111