FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30670862
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC6231352.0316840133153087TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC688650.042.69762444
TCTCGTA681350.042.47085641
TCGTATG691650.042.43609643
CTCGTAT684950.042.42748342
GATCGGA691450.042.3688131
CGTCTGA700900.042.34192316
GATCTCG680050.042.26099839
ACGTCTG703800.042.182615
ACACGTC705350.042.10508313
ATCGGAA709000.042.1033632
ATCTCGT687700.041.82919740
TCGGAAG713450.041.797523
CACGTCT716650.041.46300514
CGGAAGA719100.041.4415134
GCACACG718000.041.4058811
CACACGT718800.041.3262712
AGTGATC721400.039.9693136
GTGATCT730350.039.41708437
GTCACAC749400.039.35581229
GAACTCC755100.039.258421
Produced by FastQC (version 0.11.2)