Basic Statistics
Measure | Value |
---|---|
Filename | HFCWMBGXY_n01_sv124.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 40467126 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC | 2571597 | 6.354780421026193 | TruSeq Adapter, Index 4 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 276200 | 0.0 | 43.346195 | 1 |
ATCGGAA | 281685 | 0.0 | 43.288315 | 2 |
TCGGAAG | 282215 | 0.0 | 43.18041 | 3 |
CGTATGC | 278980 | 0.0 | 43.05233 | 44 |
CGTCTGA | 282095 | 0.0 | 43.030956 | 16 |
ACACGTC | 282420 | 0.0 | 43.013657 | 13 |
ACGTCTG | 282395 | 0.0 | 42.99156 | 15 |
CGGAAGA | 283400 | 0.0 | 42.967255 | 4 |
GCACACG | 283915 | 0.0 | 42.807972 | 11 |
TCGTATG | 280960 | 0.0 | 42.739582 | 43 |
AATCTCG | 276195 | 0.0 | 42.730938 | 39 |
CACACGT | 284330 | 0.0 | 42.729355 | 12 |
CTCGTAT | 277500 | 0.0 | 42.727264 | 42 |
TCTCGTA | 277060 | 0.0 | 42.72451 | 41 |
CACGTCT | 284480 | 0.0 | 42.707962 | 14 |
ATCTCGT | 277500 | 0.0 | 42.59018 | 40 |
ACTGACC | 284850 | 0.0 | 42.054058 | 32 |
ACCAATC | 279675 | 0.0 | 42.046207 | 36 |
GAACTCC | 288535 | 0.0 | 41.92311 | 21 |
GACCAAT | 283760 | 0.0 | 41.909798 | 35 |