FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40467126
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC25715976.354780421026193TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2762000.043.3461951
ATCGGAA2816850.043.2883152
TCGGAAG2822150.043.180413
CGTATGC2789800.043.0523344
CGTCTGA2820950.043.03095616
ACACGTC2824200.043.01365713
ACGTCTG2823950.042.9915615
CGGAAGA2834000.042.9672554
GCACACG2839150.042.80797211
TCGTATG2809600.042.73958243
AATCTCG2761950.042.73093839
CACACGT2843300.042.72935512
CTCGTAT2775000.042.72726442
TCTCGTA2770600.042.7245141
CACGTCT2844800.042.70796214
ATCTCGT2775000.042.5901840
ACTGACC2848500.042.05405832
ACCAATC2796750.042.04620736
GAACTCC2885350.041.9231121
GACCAAT2837600.041.90979835