FastQCFastQC Report
Mon 8 Aug 2016
HFCWMBGXY_n01_sv123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCWMBGXY_n01_sv123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35526367
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC13006943.6612074631779823TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1370850.043.10274544
GATCGGA1378100.042.8739131
CGATGTA1373850.042.86939634
CACCGAT1379300.042.80794531
ACCGATG1377500.042.80748732
CTCGTAT1375700.042.7686242
CGTCTGA1395700.042.76327516
TCTCGTA1373850.042.74935541
ACACGTC1398850.042.7451913
TCGTATG1381050.042.7413943
ATCGGAA1403500.042.7373282
CCGATGT1378000.042.71422633
GTCACCG1383700.042.71181529
ACGTCTG1400300.042.6383315
TATCTCG1378150.042.6000739
TCGGAAG1410450.042.505923
ATCTCGT1383200.042.46992540
TCACCGA1391600.042.45979730
CGGAAGA1415200.042.2932434
GCACACG1413750.042.2821711