Basic Statistics
Measure | Value |
---|---|
Filename | HFCWMBGXY_n01_sv123.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 35526367 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 1300694 | 3.6612074631779823 | TruSeq Adapter, Index 2 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 137085 | 0.0 | 43.102745 | 44 |
GATCGGA | 137810 | 0.0 | 42.873913 | 1 |
CGATGTA | 137385 | 0.0 | 42.869396 | 34 |
CACCGAT | 137930 | 0.0 | 42.807945 | 31 |
ACCGATG | 137750 | 0.0 | 42.807487 | 32 |
CTCGTAT | 137570 | 0.0 | 42.76862 | 42 |
CGTCTGA | 139570 | 0.0 | 42.763275 | 16 |
TCTCGTA | 137385 | 0.0 | 42.749355 | 41 |
ACACGTC | 139885 | 0.0 | 42.74519 | 13 |
TCGTATG | 138105 | 0.0 | 42.74139 | 43 |
ATCGGAA | 140350 | 0.0 | 42.737328 | 2 |
CCGATGT | 137800 | 0.0 | 42.714226 | 33 |
GTCACCG | 138370 | 0.0 | 42.711815 | 29 |
ACGTCTG | 140030 | 0.0 | 42.63833 | 15 |
TATCTCG | 137815 | 0.0 | 42.60007 | 39 |
TCGGAAG | 141045 | 0.0 | 42.50592 | 3 |
ATCTCGT | 138320 | 0.0 | 42.469925 | 40 |
TCACCGA | 139160 | 0.0 | 42.459797 | 30 |
CGGAAGA | 141520 | 0.0 | 42.293243 | 4 |
GCACACG | 141375 | 0.0 | 42.28217 | 11 |