FastQCFastQC Report
Sat 2 Jul 2022
HFCVLBGXM_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCVLBGXM_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429641587
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCTTT10799040225.134997464758925No Hit
CAAGTCCA10620611124.719699911172704No Hit
TCTTAGGC8889757220.69110037059797No Hit
GTGAGAAG8360831219.46001377189774No Hit
GGGGGGGG242625485.647160036209438No Hit
AGCCCTTA8376030.19495389304574No Hit
CAAGTCAA4908240.11424033772596599No Hit
TCTTAGCA4739900.1103221881544721No Hit
AGCCTTTA4720760.10987670055319855No Hit
TCTAGGCA4332210.10083311604563085No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)