FastQCFastQC Report
Sat 2 Sep 2023
HFCNFDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCNFDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026830741
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG22900338122.30195998777563Illumina Single End PCR Primer 1 (100% over 8bp)
TCACGCGT208619802.0316863497564492No Hit
CACTAGAC189010761.840719725783901No Hit
GTGACGCA159325731.5516260240206423No Hit
ACGCTATC158952931.5479954354035061No Hit
CATACTAG149252451.4535253381160704No Hit
TACTCCTT144535331.4075867056652525ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
TGCTGCTT137563971.3396946985248077No Hit
AGGCTTAG134264151.3075587303633327No Hit
AGAGGATA130346741.2694082363862536No Hit
TATGCAGT128262581.2491112203661616No Hit
TGCAACCT125389491.2211310490946823No Hit
AGTGTAGA124712141.2145345383655592Illumina Single End PCR Primer 1 (100% over 8bp)
TTGGTGCT120529931.173805235735536No Hit
TCTACTCT119368931.1624986011204743No Hit
TGGTTCAC113054901.1010081358676425No Hit
TTCTTCCG112936731.099857313290156No Hit
CACGACAT108156531.0533043634306232No Hit
CTCCTTAC106952951.0415830548259755No Hit
GCATTGTC102490390.9981235067055712No Hit
GAAGGACT95813870.93310285886737No Hit
ATAGAGAG93633870.9118724855160915ABI Solid3 Adapter B (100% over 8bp)
CTCTAGCT84622860.824116932042649No Hit
ATGCCTCA83528140.8134557786870934No Hit
ATGTCAAT83484890.813034579766248RNA PCR Primer, Index 15 (100% over 8bp)
TTATGGCC83423510.8124368181532657No Hit
CAGTTCCT83289250.8111292998385212No Hit
TCAGGCAA81477450.793484717068867No Hit
CTGACGTG80383300.7828291147742333No Hit
TTGTGCGT80084650.7799206510121418No Hit
TCATTACA79789280.777044130197111No Hit
AGTTGGCT78034630.7599561143251748No Hit
GTAGATCG77086150.7507191489507616No Hit
GTTCCTAG76778840.7477263480174675No Hit
TGAACCGA76067430.740798137051489No Hit
TGTTACGG75827760.7384640620142867No Hit
GTCGGTAA74242840.7230289962657048No Hit
TCTCGAAC73609340.7168595276794503No Hit
ACCTAGTA73375310.714580378929267No Hit
GCGATCTA72793500.7089143039203186No Hit
AACCGTGA72397660.7050593355774883No Hit
CCATCTTA70386890.685477042997878No Hit
TTCAGCAC70231840.6839670570400268No Hit
GTTCGACA70160380.6832711292970532No Hit
GAGCTAAC69163050.6735584282629108No Hit
TAGCGACG68006100.6622912353965063No Hit
GTTAGTGC67566130.6580064980738631No Hit
GTTGTGAC66912160.6516376782295807No Hit
CGAGAGTT66522350.6478414342680845TruSeq Adapter, Index 13 (100% over 8bp)
CCTATCAC63663600.6200009160029617No Hit
TTCTCTGC62949110.613042709830597No Hit
CCAATGCT62860150.612176354778611No Hit
CGTACGAT61074960.5947909189056895RNA PCR Primer, Index 22 (100% over 8bp)
GGGGGGGG60503380.5892244708322382No Hit
TAGCACAG60282790.5870762102553765No Hit
TGGCGGTC59747000.5818583103756143No Hit
TCCAGAGA58374220.5684892131603996No Hit
TATCTGAC57907300.5639420177818771No Hit
CATGGCCA57745550.5623667825114325No Hit
ACGGTGTC56956620.5546836272600452No Hit
AATCTCTC56918580.5543131669837688No Hit
TAACCGCG56751700.552687972165025No Hit
TCCTCAAG54697690.5326845780516032No Hit
GAGTAGCC54669200.5324071223925306No Hit
GTGACTCT53124770.5173663767434871No Hit
TATAGTAG52093010.5073183721522416No Hit
ACTCGCTA51426390.5008263577103015No Hit
CTCGATGA51190700.4985310427125204No Hit
ACACGATC51080840.4974611487600564No Hit
CTCGTCGA50836040.4950771141745551No Hit
TAGTAACG50314310.4899961404642053No Hit
GTGTATAT49759970.4845975876368898No Hit
GTCTCGTA49588930.48293187981211794TruSeq Adapter, Index 14 (100% over 8bp)
CGAGACGT46457840.45243912307062495No Hit
GTCTGAGG45982060.4478056427802252No Hit
GGAGTTCC45770420.4457445435985442RNA PCR Primer, Index 1 (100% over 8bp)
CTACACTA44920020.43746275025087117No Hit
ACTAGAGC44256490.4310008284023511No Hit
CACGTCGT44243310.43087247229190623No Hit
TCAGTGTC42628630.4151475827309693No Hit
CGTACGCA42174140.4107214394353626No Hit
AGATATCC41899530.4080470941023376No Hit
AGCTCTAG41661760.4057315226015424No Hit
CGTAAGGA41593580.4050675378056295No Hit
GAAGTCAG41085520.4001196921703769No Hit
CACTCACG39661780.38625431063131754No Hit
CCACGCGT39361800.3833328943937412No Hit
TCTCAGTG37599990.3661751494056604No Hit
CTGTTGGA36432710.3548073557334217No Hit
CAGATAGT33708670.32827873819956077No Hit
TTCCGTCT32382590.31536443843182527TruSeq Adapter, Index 14 (100% over 8bp)
GCTCACAA31700480.3087215714746507No Hit
GGTACCTT30335940.2954327211752224No Hit
GCATCCAT28600550.27853227273023373No Hit
GAGGCTAT28589480.2784244652839041No Hit
ACACGCAG27940790.2721070657934266No Hit
GGCTAACA27632420.26910394183455794No Hit
CACCTACA27178640.26468471301834545No Hit
CCTGAACT26903440.26200462185033085No Hit
TTCCACGA25062230.2440736238145017No Hit
TTCACGCA23879040.2325508873716121No Hit
GGACGTAT22200090.2162000913449474No Hit
GACCATTG22106490.21528854870931452No Hit
GACTTCGA21018230.20469030737754276No Hit
GGTCTATG20743760.2020173254629898No Hit
GTTAACCG18994900.18498569668357834No Hit
TGATCTCG16271040.15845883211632442Illumina PCR Primer Index 5 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)