FastQCFastQC Report
Sat 2 Sep 2023
HFCNFDRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCNFDRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026830741
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGG24793553924.14570669734166No Hit
TTCACGCA285116922.776669110259916No Hit
TGGCTTCA275125772.679368264063298No Hit
AAGGACAC264671542.5775576191091067No Hit
CACTAGCT224538682.1867156000932386Illumina PCR Primer Index 10 (100% over 8bp)
AGCACCTC221696172.15903323837088No Hit
ACAGATTC220616982.1485233270786934No Hit
AGCCATGC214105772.085112584294942No Hit
TATCAGCA207307512.018906346708216No Hit
AATCCGTC200375741.9513998948342741No Hit
TCTTCACA198787201.9359295749794854No Hit
GCGACTAA178898591.7422403017051864No Hit
TCCGTCTA177304821.726719048431761No Hit
ATCATTCC175085941.7051100342933734No Hit
AACCTCCA172630751.681199667161114No Hit
ACACGACC165304681.6098532445475353No Hit
ACAGTGGT165076441.6076304828898769No Hit
GGTTGCAA159101341.5494407563709667No Hit
GAACCACA149687431.4577614793088864No Hit
AGTCGCAG148273891.443995432544223No Hit
CCTAAGAC139110821.354759011836012No Hit
ACGTACGT130025751.2662822099908286No Hit
GCATAAGG114193751.112099058203011No Hit
ACTACGAC112963381.1001168497350235No Hit
GCGATACG111818991.0889719749829734No Hit
ACCGGTAA111772641.0885205860816745No Hit
CATCTTCG107461561.0465362567480827No Hit
ACTGAGCG88829940.8650884362255375No Hit
TGAGTACG87604020.8531495649875561No Hit
CATTACGG86985600.8471269560481536No Hit
CGATCAGT84416410.8221063767314695No Hit
TACTAGGT84057310.8186092083505317No Hit
ACGCTACT82318920.8016795437954267No Hit
CGAGCGAC77592810.7556533604012933No Hit
ACGTGCGC75772210.737923077041964No Hit
ATGCGCAG72068480.7018535492014453No Hit
ACTCACTG71047730.6919127677343272No Hit
CGAGCTAG70701380.6885397678213844No Hit
CGTGTACA70239500.6840416555078574No Hit
ATAGCGCT69095690.6729024292037631No Hit
TAGTCTCC68172050.6639073732211139No Hit
CGAGAGTT67188810.654331890517485TruSeq Adapter, Index 13 (100% over 8bp)
GATTCGGA66949870.6520049247337444No Hit
GCGTATAC66709330.6496623770246084No Hit
TAGCGCTC66586350.6484647112839018No Hit
ATAGTACC61345570.5974263094252259No Hit
GTCTGCTA60966540.5937350486860814No Hit
GTAACGAG59853540.5828958718328827No Hit
CAGTAGGT56984790.5549579665340385No Hit
CGCGATAT56156450.546891008982755No Hit
GACATAGT55354170.5390778420413497No Hit
TCTAGACT54786740.5335518095868927No Hit
CGAAGTAT53255050.5186351350188103No Hit
TCTCTATG49682170.4838399165145368ABI Solid3 Adapter B (100% over 8bp)
CGGAGCCT49153460.4786909666546495No Hit
TGCTCGTA47940240.46687577694949434No Hit
AACGCTGA47276870.46041541329351376No Hit
TAGCAGCT46812840.4558963627677369No Hit
GATCTACG41243330.4016565569495352No Hit
CTCGACTT40377170.39322128163671716No Hit
TACGCTGC36437500.3548540041225743No Hit
GTTACAGC35476050.3454907277654244No Hit
CTGCGTAG34123020.3323139699418095No Hit
CTCTAGAG32385600.31539375192897545No Hit
CGTAGCGA31493770.3067084841005943No Hit
ATGAGCTC31162450.3034818568993349No Hit
AGCATACC30509020.29711829595487343No Hit
TAACGTCC28249730.27511574081321744No Hit
ACTCGCTA27224570.2651320116642281No Hit
ATCTCAGG24954710.24302651842792855No Hit
GTCTATGA24366910.23730210858578105No Hit
GCGTAGTA24281590.23647120241406952No Hit
CGTCATAC21818300.2124819517844957No Hit
TCAGTCTA21765380.21196657960204174No Hit
GGAGCTAC20760340.20217879316489998No Hit
GCTCATGA14947310.14556741830151343No Hit
TATAGCGA14574550.14193721923251226No Hit
TCCTCATG12550670.12222725225169316No Hit
CTATCGTG12550190.12222257767407453No Hit
GAGCTCGA11567850.1126558598034805No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)