FastQCFastQC Report
Sat 2 Sep 2023
HFCNFDRX3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCNFDRX3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026830741
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG22149227221.57047536230706No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC341656423.327290529569371No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC176052241.7145205433618784No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTAC143427601.396798851778825No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAAC121735841.1855492355190407No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGATAAC91983010.8957952496671504No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCGTCTGTCTCTTATACACATC83768380.8157954047852177No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTAC72541230.7064575212206273No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAAC66710540.6496741608556886No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAAC60650580.5906580079686181No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCGTCTGTCTCTTATACACATC56849500.5536404173548208No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGC56171200.5470346548574941No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGCGC53310070.5191709584783458No Hit
GAGTAACCGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGATAAC49877430.4857414957349821No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCGTCTGTCTCTTATACACATC48635490.473646610468979No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGT43141070.4201380838869918No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGTTGGGCGC42872630.41752382635377294No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCTAC41230090.401527616516888No Hit
GAGTAACCGGATTAGATACCCGGGTAGTCCGTCTGTCTCTTATACACATC38009710.37016529095129613No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGTTGGGCGC37907930.3691740857220791No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATGAC37134960.3616463601765113No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGC34699090.3379241447934017No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAAC34564690.3366152630601853No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGGTGC33197450.3233001182616522No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCAAC32973170.3211159218693473No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATGC32169180.31328610174517557No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGGCAC31726610.30897604379376487No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATAC28451020.2770760444149967No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGC26882580.26180147249798785No Hit
GAGTAACAGGATTAGATACCCTTGTAGTCCGTCTGTCTCTTATACACATC26636220.25940224553522595No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGCGC26030600.25350429199898683No Hit
GAGTAACAGGATTAGATACCCTCGTAGTCCGTCTGTCTCTTATACACATC25144210.2448720027169502No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGAAC24814230.24165842537820945No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAAC24738570.24092159508107286No Hit
GAGTAACAGGATTAGATACCCGTGTAGTCCGTCTGTCTCTTATACACATC23118770.22514684335887056No Hit
GAGTAACAGGATTAGATACCCGCGTAGTCCGTCTGTCTCTTATACACATC22920290.223213905513567No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGT22283710.21701444172092627No Hit
GAGTAACAGGATTAGATACCCTAGTAGTCCGTCTGTCTCTTATACACATC22227300.21646508146370347No Hit
GAGTAACCGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCTAC21824110.21253853365108788No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACACCGTAAACGTTGGGCGC21823430.2125319113327948No Hit
TGAACCGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCTACTA21033660.20484057557057497No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGTCAAC20883650.2033796726777193No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCGC20625790.20086845062617775No Hit
TGAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTACTA20611840.2007325957141363No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGCGC20436110.19902121337054907No Hit
CTGTAAAGATAAATGGGTCATCTAATCCTGTTACTCCTGTCTCTTATACA18887480.18393956516734242No Hit
CTGTAAAGATAAATGGGTCATCTAATCCGGTTACTCCTGTCTCTTATACA18591730.1810593436450302No Hit
GAGTAACCGGATTAGATACCCTTGTAGTCCGTCTGTCTCTTATACACATC18459900.17977549037948018No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCTGTAAACGTTGGGCGC18107950.17634795372765336No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGT17919610.17451376633454335No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATGAC17514920.17057261046686956No Hit
GAGTAACCGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCAAC17429300.16973878268414638No Hit
GAGTAACCGGATTAGATACCCATTTATCTTTACAGCTGTCTCTTATACAC17276200.1682477871978708No Hit
CTGTAAAGATAAATGGGTATCTAATCCTGTTACTCCTGTCTCTTATACAC17159400.16711030664400414No Hit
GAGTAACCGGATTAGATACCCTCGTAGTCCGTCTGTCTCTTATACACATC16809640.1637040977525623No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGC16733430.16296191116857106No Hit
GAGTAACAGGATTAGATACCCGAGTAGTCCGTCTGTCTCTTATACACATC16669150.1623359073158095No Hit
TGAACCGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGATAACTA16120500.15699276771068152No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGAAC16103270.15682496985157945No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGGTGC16044550.15625311318956703No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGAAGC16018280.15599727745198078No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGGCAC15347140.1494612440707986No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATGC15003240.14611210398111757No Hit
GAGTAACCGGATTAGATACCCTAGTAGTCCGTCTGTCTCTTATACACATC14436420.1405920121357177No Hit
TGAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTACTA14429510.14052471769541616No Hit
GAGTAACAGGATTAGATACCCATTTATCTTTACAGCTGTCTCTTATACAC14429390.1405235490510115No Hit
TGAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAACTA14271920.13898999543099966No Hit
CTGTAAAGATAAATGGGTCATCTAATGAGTATGGATCTGGGATTGATCCG14099710.1373128933232824No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGAATGC13940450.13576190742423438No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATAC13877370.1351475900155194No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACTATGAGAGC13843850.13482114867848508No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCGAC13623840.1326785365495792No Hit
TGAACAGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCTACTA13620250.1326435746044732No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCGTAAACGTTGGGCGC13412570.1306210406881459No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAAC12990010.12650585419121183No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGAAC12777650.12443774314310288No Hit
GAGTAACAGGATTAGATACCCTGTAGTCCGTCTGTCTCTTATACACATCT12543950.12216180816503233No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGAATGT12423120.12098508063657593No Hit
GAGTAACCGGATTAGATACCCGTGTAGTCCGTCTGTCTCTTATACACATC12362900.12039861591950528No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGGGCAC12254150.11933953192778439No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGAAC12200050.11881266807535129No Hit
CTGTAAAGATAAATGGGTATCTAATCCGGTTACTCCTGTCTCTTATACAC11862100.1155214732707345No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGCGC11808190.11499645977194212No Hit
GAGTAACCGGATTAGATACCCGCGTAGTCCGTCTGTCTCTTATACACATC11790700.11482612984996327No Hit
GAGTAACCGGATTAGATACCCTGTCTCTTATACACATCTCCGAGCCCACG11731700.11425154635100665No Hit
GAGTAACCGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGTCAAC11654300.11349777071000254No Hit
GAGTAACAGGATTAGATACCCTGTCTCTTATACACATCTCCGAGCCCACG11380810.1108343327247543No Hit
GAGTAACAGGATTAGATACCCCCGTAGTCCGTCTGTCTCTTATACACATC11185770.1089348960190509No Hit
GAGTAACCGGATTAGATACCCTGTAGTCCGTCTGTCTCTTATACACATCT11126650.10835914387568964No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACACCGTAAACGTTGGGCGC11125690.10834979472045236No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTGC10950190.10664065227864072No Hit
GATAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAACT10717560.10437513771317838No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCGC10427360.10154896599458157No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCTGTAAACGTTGGGCGC10341700.10071474866372354No Hit
TGAACAGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGATAACTA10288030.10019207245374046No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)