FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l02n01_bgm31.3510000006558c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l02n01_bgm31.3510000006558c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25414411
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC12475444.908805480481133TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1422500.042.2495271
ATCGGAA1423900.042.162312
CTCGTAT1374400.041.91960542
TCTCGTA1374850.041.90097441
TCGTATG1378550.041.88317543
CGTATGC1378750.041.87709844
GTATGCC1380250.041.83973745
AATCTCG1372450.041.82982639
TCGGAAG1433750.041.726723
ATCTCGT1384200.041.5773140
CGGAAGA1438400.041.4917224
GTAATCT1382900.041.48444737
TAATCTC1397400.041.1071238
TTGTAAT1401350.041.01854735
TGTAATC1399500.041.01328736
CGTCTGA1433650.040.92770816
ACACGTC1438800.040.9172513
ACGTCTG1442500.040.70156515
CTTGTAA1416200.040.61075634
CACGTCT1453650.040.4497314
Produced by FastQC (version 0.11.2)