FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l02n01_bgm30.35100000065562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l02n01_bgm30.35100000065562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28737211
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2604660.9063718813909951TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT311550.039.4222642
TCTCGTA311800.039.3762241
TCGTATG314200.039.1184143
CGTATGC314900.039.06002844
ATCTCGT319000.038.4804240
CATCTCG309800.038.30854839
GTATGCC328100.037.55715645
ATCGGAA346500.036.6794622
GATCGGA344950.036.624131
GATCATC331550.035.89725536
TCGGAAG354150.035.8871543
CGTCTGA352250.035.74859216
ACGTCTG354950.035.4449715
CGGAAGA358600.035.3853454
ACACGTC361400.034.90575413
AGATCAT346750.034.36921735
ACCAGAT357850.033.4351632
AGTCACC367300.033.1324128
GTCACCA362150.033.1313629
CACGTCT381800.033.02302614
Produced by FastQC (version 0.11.2)