FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l02n01_bgm29.35100000065556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l02n01_bgm29.35100000065556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30602002
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC17004185.556558031726159TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1819000.044.809681
ATCGGAA1825500.044.6669542
TCGGAAG1830500.044.4761163
CGGAAGA1835350.044.336524
ACACGTC1823600.044.19778413
CGTCTGA1824200.044.0956816
ACGTCTG1827500.044.0443715
GCACACG1838950.043.93162511
CACGTCT1836500.043.87262714
CACACGT1844300.043.77735512
CGCCAAT1819450.043.6210333
GTCACGC1825350.043.5724829
ACGCCAA1825500.043.5245332
TATCTCG1817400.043.46961239
AGTCACG1830500.043.4440328
TCACGCC1830850.043.4231530
TCTCGTA1822150.043.39711441
CACGCCA1831400.043.37816631
CTCGTAT1823150.043.36837442
ATCTCGT1827050.043.25363540
Produced by FastQC (version 0.11.2)