FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l02n01_bgm28.3510000006553c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l02n01_bgm28.3510000006553c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24392049
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC596990.24474778646107181TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT71500.038.86123342
TCTCGTA72100.038.5378441
GATCTCG71850.038.26469839
CGTATGC73000.038.18599744
GATCGGA75800.038.0003171
TCGTATG73700.037.73172443
ATCGGAA77000.037.458572
ACACGTC76050.037.42356513
CGTCTGA76500.037.29165616
ATCTCGT77750.035.67946640
ACGTCTG81050.035.1981715
TCGGAAG83200.034.8294333
CGGAAGA85800.033.852664
CACGTCT87800.032.4921614
GCACACG93550.030.66339711
CACACGT96550.029.82713712
GTATGCC95750.029.16007845
AGTGATC111400.025.00283836
GTGATCT121500.022.94293237
GTCACAC127050.022.3657529
Produced by FastQC (version 0.11.2)