FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l02n01_bgm27.3510000006552f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l02n01_bgm27.3510000006552f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26582664
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1869450.7032590864482205TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA219100.041.280261
CGTCTGA216450.041.0888416
ATCGGAA220950.041.005522
TCTCGTA212950.040.86620341
CTCGTAT213300.040.77805742
ACACGTC218850.040.7718913
ACGTCTG219400.040.5671415
TCGTATG215250.040.5236143
AATCTCG214900.040.42202439
TCGGAAG225450.040.147133
CGTATGC217700.040.12956644
ATCTCGT219400.039.68531840
CGGAAGA227900.039.6661724
CACGTCT229300.038.8843414
GCACACG233900.038.2542811
CACACGT239900.037.30690812
GTATGCC238450.036.65634545
ACCAATC247900.034.98666436
GACCAAT252650.034.4803435
ACTGACC259400.033.73055632
Produced by FastQC (version 0.11.2)