FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l02n01_bgm26.35100000065512.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l02n01_bgm26.35100000065512.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28232388
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3887921.3771134060639858TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA423500.042.89340216
GATCGGA431300.042.83771
CGATGTA416550.042.81539534
ATCGGAA432800.042.746272
ACACGTC427400.042.65466313
ACCGATG418750.042.63881332
CACCGAT420450.042.57878531
ACGTCTG428050.042.49528515
CCGATGT420350.042.4658133
TCTCGTA421250.042.42307741
CTCGTAT421450.042.4029442
TATCTCG422000.042.3314639
GTCACCG424750.042.2695629
CGTATGC424350.042.23510444
TCGTATG424350.042.1873943
TCGGAAG439800.041.9891743
CGGAAGA439950.041.908374
ATCTCGT427600.041.78255540
TCACCGA430350.041.66201830
CACGTCT438300.041.5579614