FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_wt2.3510000006563b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_wt2.3510000006563b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10589525
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG596890.5636607874290868TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG127350.034.25568434
GTTCCGT128950.033.88298835
GATCGGA84200.033.1318241
CCGTATC130350.033.12209338
TATCTCG132000.032.57171241
TCCAGTC135400.032.33736425
TCCGTAT135150.032.22872537
GTCACAG136150.032.1254229
AGTCACA136800.032.0714628
CAGTCAC136900.031.9988827
CGTATCT134650.031.99751339
CTCGTAT134600.031.9428444
ACACGTC139250.031.8614513
ACGTCTG139300.031.76925515
CACACGT139550.031.69621812
CACGTCT140300.031.54281814
ATCTCGT136450.031.5094642
CCAGTCA139600.031.41222626
GCACACG140950.031.3494711
AGCACAC140500.031.32177210
Produced by FastQC (version 0.11.2)