FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_wt1.35100000065599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_wt1.35100000065599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8111452
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC690600.8513888758757372TruSeq Adapter, Index 5 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG162620.20048198522286761TruSeq Adapter, Index 5 (100% over 51bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC110470.13619016669272035TruSeq Adapter, Index 5 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA102000.037.532971
CTCCCGT15350.034.89276523
GACCGGA9900.032.7560461
TCGGGAG11600.032.196392
AGTCACA189000.031.93958928
TCCAGTC186900.031.818525
CAGTCAC190200.031.56083127
CCAGTCA189000.031.52312926
CACACAG192500.031.21745931
GTCACAC193150.030.90349829
GATCTCG193800.030.82230839
AGTGATC195700.030.67251436
ACTCCAG194150.030.61243223
GTATGCC201600.030.5454645
CTCGTAT197800.030.38099742
ATCTCGT197600.030.36620140
GTGATCT197400.030.28299137
CTCCAGT195950.030.26269124
CACGTCT202700.030.10165414
CACAGTG202650.030.07577133
Produced by FastQC (version 0.11.2)