FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_r2.3510000006567e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_r2.3510000006567e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10115109
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG23400.023.74867632
CGTCCGC23600.023.54741733
AGTCACG24550.022.82029328
GTCACGT26600.021.23054529
CCGCACA26850.020.78096636
GCACACG28200.020.10526311
CACACGT28700.019.83339112
CATCTCG29200.019.49377841
ACACGTC29300.019.42724613
TCACGTC29900.018.81212630
ACGTCTG31200.018.388415
CACGTCC31700.017.67251831
CACGTCT32750.017.58680514
GTCCGCA33100.017.53678734
TCCAGTC32050.017.48030325
AGCACAC31900.017.4206610
CAGTCAC32150.017.28579327
CGTCTGA35550.016.58135616
GAGCACA34700.016.4688249
GCACATC34950.016.28664638
Produced by FastQC (version 0.11.2)