FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_r1.35100000065655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_r1.35100000065655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13777135
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26300.021.38666311
ACACGTC28300.019.47766313
CACACGT29000.019.16274512
ACGTCTG30800.018.11582815
CACGTCT31450.017.66987614
AGCACAC32150.017.63511710
GAGCACA34000.016.940259
CACATGT33550.016.49680331
TCACATG33300.016.2831330
TCCAGTC34650.015.71422525
GAACTCC34700.015.62946321
CAGTCAC35900.015.22941427
AGTCACA37050.014.99961628
CGTCTGA39000.014.59530216
GTCACAT37700.014.56180229
CTCGTAT36550.014.46574644
GTCTGAA41150.013.77805417
ATGTCAG40550.013.70450434
ATCTCGT38650.013.38861142
CCAGTCA41650.013.18093426
Produced by FastQC (version 0.11.2)