FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_mms2.3510000006568b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_mms2.3510000006568b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7914325
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG937041.1839796824113238TruSeq Adapter, Index 19 (97% over 40bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG146770.1854485379359579TruSeq Adapter, Index 19 (97% over 40bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG92980.1174831713380484TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA105900.041.695181
AGTCACG178700.040.00119828
CGATCTC180550.039.1155540
GTCACGT183400.038.93927829
TCACGTG183200.038.92038330
CACGTGA184700.038.6751931
CAGTCAC186150.038.54533427
GCACACG189250.038.29221311
TCCAGTC187100.038.19425225
GATCTCG185850.038.0848141
ACACGTC192050.037.85107813
CACACGT192050.037.85107812
ACGATCT187150.037.79621539
CCAGTCA189550.037.74710526
AGCACAC193050.037.5967410
AACGATC188700.037.56921838
CACGTCT193400.037.54033314
ACGTCTG193050.037.53846715
CGTCTGA193100.037.5287516
ACTCCAG193150.037.09695423
Produced by FastQC (version 0.11.2)