FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_mms1.351000000655a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_mms1.351000000655a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9916135
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1211201.2214436370622224TruSeq Adapter, Index 6 (100% over 51bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC182040.18357959023349318TruSeq Adapter, Index 6 (98% over 51bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC116130.11711216113939554TruSeq Adapter, Index 6 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA144150.040.0808071
CACGCCA235650.039.17299331
AGTCACG236450.038.8899828
GTCACGC238850.038.51785329
TATCTCG238150.038.346139
TCACGCC241500.038.23474530
CGCCAAT243150.038.07573333
ACGCCAA245200.037.70234332
GCACACG248400.037.48836511
CACACGT250150.037.26208512
ACACGTC251000.037.22553313
TCCAGTC247550.037.16622525
CACGTCT251100.037.15694414
ACGTCTG251400.037.10365715
GTATGCC249150.037.0959645
CCAGTCA249000.036.9750426
GCCAATA249900.036.93924334
AGCACAC253350.036.7292710
CAGTCAC251500.036.67906627
CTCGTAT250550.036.672842
Produced by FastQC (version 0.11.2)