FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_input_wt.35100000065549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_input_wt.35100000065549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9966301
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC615180.6172601048272575TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA74250.038.8463251
CCGATGT132800.034.17106633
CGATGTA136300.033.35962334
GTCACCG136600.033.1229929
CACCGAT137700.033.0041231
CAGTCAC138750.032.7723827
ACCGATG138950.032.67482832
CCAGTCA140350.032.3346526
GCACACG141950.032.3329611
TCCAGTC140150.032.3011725
TCACCGA141700.031.99419230
AGTCACC142600.031.80836328
ACACGTC144550.031.76695613
TATCTCG142950.031.74478739
CACACGT144300.031.74403612
ACGTCTG145150.031.57364315
CGTCTGA147100.031.21627416
GTATGCC145750.031.16628345
CACGTCT147200.031.14921614
TATGCCG41200.031.12682745
Produced by FastQC (version 0.11.2)