FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_input_r.35100000065604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_input_r.35100000065604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15369301
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20750.017.34832811
CACACGT24800.015.60387712
ACACGTC25150.015.11835213
ACGTCTG26100.014.1370615
CACGTCT28400.013.3090414
GATCGGA19550.012.6700311
CGTCTGA29700.012.57498316
CAGTCAC30500.012.24554627
AGCACAC29800.012.15529210
TCCAGTC32100.011.77562625
GTCACAG31650.011.58722829
CACAGTC33300.011.21552631
GAGCACA33150.011.1305369
GAACTCC34400.010.99030621
CCAGTCA33200.010.97859726
CTCGTAT36600.010.38877244
AGTCACA36950.010.29060228
ATCTCGT38000.09.82834242
AGAGCAC37550.09.7663768
TCTCGTA41600.09.08603843
Produced by FastQC (version 0.11.2)