FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_input_mms.3510000006557f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_input_mms.3510000006557f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7662640
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC3369754.397635801760229TruSeq Adapter, Index 4 (100% over 51bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC509530.6649535930175501TruSeq Adapter, Index 4 (98% over 51bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC351150.4582624265266279TruSeq Adapter, Index 4 (98% over 51bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC230490.3007971143104726TruSeq Adapter, Index 4 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG145720.19016944551747178TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA369850.044.0430341
GAGCGGA60200.040.359831
AGTCACT631050.039.90046328
CAGTCAC633100.039.75045827
TCCAGTC630900.039.72171425
ACTCCAG633050.039.68065623
CCAGTCA632950.039.6176926
CTCCAGT633350.039.58039524
ACTCCCG37150.038.7692622
CGGAAGA587700.038.4200554
AACTCCA656800.038.4060922
GTCACTG658250.038.32298729
GACCAAT657800.038.3137535
ACTGACC661700.038.24773832
ACCAATC658400.038.14898736
CTGACCA662650.038.13858433
AATCTCG656350.038.1241739
GCACACG669300.038.11596311
ATCTCGT658100.038.10825740
TCTCGTA660050.038.05362341
Produced by FastQC (version 0.11.2)